cre lox recombination pptamir and baba relationship in america

the loxP sites will delete the gene A novel maize variety designated X00R808 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. orientation of the two loxP, one can create a gene inversion, translocation, or deletion. The asymmetry in the core sequence gives the loxP site directionality, and the canonical loxP sequence is ATAACTTCGTATA-GCATACAT-TATACGAAGTTAT. Maize hybrid X13C733 Patent | Application Number: 13396674 Expression of Cre recombinase by AtRps5apro or AtDD45pro enabled Cre/lox-mediated recombination at an early embryonic developmental stage upon crossing, activating transgenes in the hybrid cowpea and tobacco. This technique is called recombinase-mediated cassette exchange and is a very convenient and time-saving way for genetic manipulation. Missirlis PI, Smailus DE, Holt RA. Site-specific recombination (SSR) involves specific sites for the catalyzing action of special enzymes called recombinases. Use of the system has greatly expanded our ability to precisely interrogate gene function in the mouse, providing both spatial and temporal control of gene expression. 2 D ). The Cre/lox-site-specic recombination system derived from bacteriophage P1 is known to be appropriate for genome modication [23-26], and its mechanism of action has been clearly described [27-29]. The https:// ensures that you are connecting to the Researchers have since reported more efficient Cre-Lox conditional gene mutagenesis in the developing T cells by the Marth laboratory in 1995. Subsequent cleavage and rejoining cause DNA strands to exchange their segments. [26][27][28] Genetic location of the floxed sequence affects recombination efficiency as well probably by influencing the availability of DNA by Cre recombinase. Therefore, researchers often use transgenic mice expressing CreERt2 recombinase induced by tamoxifen administration, under the control of a promoter of a gene that marks the specific cell type of interest, with a Cre-dependent fluorescent protein reporter. General recombination is also known as homologous recombination as it requires homologous chromosomes. Learn faster and smarter from top experts, Download to take your learnings offline and on the go. cre lox recombination ppt The SlideShare family just got bigger. This prevents non-specific actions of Cre recombinase. This recombinase comes from the P1 bacteriophage. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. Assistant Professor (Ad Hoc) For example, mating the transgenic strain in Figure 1 to a strain that expresses Cre recombinase in mammary tissue produces double transgenic offspring that express theKras oncogene only in the mammary glands (Figure 5). Thus, the orientation, and location of the loxP sites are important determinants of the recombination sites in a size-independent manner. The Cre/lox system is a well-established research tool, especially in the field of mouse transgenics. Do not sell or share my personal information, 1. This is also the catalytic site of the enzyme. See plasmid, Cre activates gene of interest by removing Stop sequences. Plasmids 101, It gives mouse researchers sophisticated control over the location and timing of gene expression. I highly recommend that you check out his blog. Siegel04.. Bacteriophage P1 uses a site-specific recombination system that is responsible for partitioning newly synthesized genomic copies during replication Abremski, Hoess.This system is composed of a 38-kD phage-encoded Cre recombinase that mediates symmetrical recombination between two 34-bp loxP sites Abremski, which are recreated after . Learn more, Download our file to copy and paste plasmid data, Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments, Basic analysis for a user-entered sequence; includes restriction sites and map, Digital collection of empty plasmid backbones from publications and commercially available sources. Cre is a 38 kDa recombinase protein from bacteriophage P1 that catalyzes recombination between LoxP sites. The Cre/lox system can be used to turn off a transgene. The mechanism of Cre recombination requires two lox sites be brought together by looping the intervening DNA. It is a member of a large family of tyrosine recombinases that includes several other familiar recombinases, such as Flp and Int. [26], Holliday junctions and homologous recombination, Implementation of multiple loxP site pairs. Hepatic Nampt Deficiency Aggravates Dyslipidemia and Fatty Liver in High Fat Diet Fed Mice. PDF Cre/lox-mediated chromosomal integration of biosynthetic gene clusters GGS Medical College/Baba Farid Univ.of Health Sciences. In our study, we mutagenized 13 bp ( 5- ATAACTTCGTATA-3) of the right RBE of the loxP sequence. The loxP mutants are created to avoid these problems.[23]. Lecture 11 - Lectures For UG-5 - studyres.com For a few examples of such strains, check out 008179 and 006481. If there exists a floxed donor sequence, the donor sequence can be swapped with the original sequence. Commonly used gene replacement strategies introduce selectable markers into the genome to facilitate selection of genetic mutations that may cause growth retardation. Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. Colorful Retro Vintage Illustration Animated Medical Technology Education Sci Quantized space time and Einstein's special theory of relativity.pdf, Doodle Astronomy Lesson XL by Slidesgo.pptx. PubMed, Orban, P.C., Chui, D., and Marth, J.D. Lox sites are directional and the two sites joined by the tetramer are parallel in orientation. The Cre or cre-recombinase protein (called so because it causes recombination) catalyzes site-specific recombination events between two DNA recognition sites. OMP targeting vector with Cre FNF (neo-selectable marker flanked by FRT sites), M71 targeting vector with IRES CreFNF (neo-selectable marker flanked by FRT sites), Cre, Puro resistance and miRNA expression. The double stranded DNA is cut at both loxP sites by the Cre protein. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling - Addgene Cre Recombinase - 1 ul (1 unit*) H20 - Xul (up to 50ul) Incubate at 37C for 30 minutes and then 70C for 10 minutes. I will also explain how to use Cre reporters). Govt. government site. Do not sell or share my personal information, 1. Cre recombinase consists of 343 amino acids, which can specifically recognize Lox sites. eCollection 2022. While Cre-lox recombination is a very useful tool, Cre expression can be toxic in some systems, notably Drosophila. KAUSHAL KUMAR SAHU Choose the incorrect statement for cre-lox excision. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. At the given specific site on the DNA, the hydroxyl group of the tyrosine in the recombinase attacks a phosphate group in the DNA backbone using a direct transesterification mechanism. 2017 by John Wiley & Sons, Inc. Keywords: 2001 Summer;5(2):81-4. doi: 10.1089/109153601300177600. Clipping is a handy way to collect important slides you want to go back to later. ERT2-Cre-ERT2 - Tamoxifen inducible. Dear Qu, Please read the following: In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to . Rafik Karaman. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). [38], A classical strategy for generating gene deletion variants is based on double cross-integration of non-replicating vectors into the genome. The Flp-FRT system and Cre-loxP induce gene recombination in a similar way. (Figure 1), recombination between Among these are loxN (GtATACcT), lox2272 (GgATACtT), and lox511 (GtATACAT). Have you used the Cre-lox system in your research? Explanation: The Cre recombinase protein of bacteriophage P1 mediates site-specific recombination at a 34 bp sequence, loxP. The cre-loxP-mediated recombination system (the "cre-loxP system") is an integral experimental tool for mammalian genetics and cell biology. FLP/FRT recombination - Oxford Reference Chief among these is Cre recombinase, discovered in the P1 bacteriophage and named as such because it c auses re combination (Cre) between specific DNA sequences referred to as loxP (locus of crossover (x) in P1) sites, each of which are 34 base pairs in length 1. Recombination/Bacteriophage P1-derived Cre-lox - parts.igem.org Common construct types include: Note: Through a partnership with genOway, Addgene is able to distribute materials containing FLEx technology. Such conditional lineage tracing has proved to be extremely useful to efficiently and specifically identify vascular smooth muscle cells (VSMCs) and VSMC-derived cells and has been used to test effects on VSMC and VSMC-derived cells in vivo.[32][33][34][35][36][37]. 2007;(178):29-48. doi: 10.1007/978-3-540-35109-2_2. Cre-LoxP Recombination - YouTube 4. [38], The P1 phage DNA when released into the host from the virion is in the form of a linear double stranded DNA molecule. What is an MTA/Who is authorized to sign? Maize hybrid X13C737 is an invention by Andrew Jon Ross, Lisbon IA UNITED STATES. Inducible Cre activation is achieved using CreER (estrogen receptor) variant, which is only activated after delivery of tamoxifen. Thus, these are site-specific, occurring only at the respective att sites. Tap here to review the details. Brault V, Besson V, Magnol L, Duchon A, Hrault Y. Handb Exp Pharmacol. Tissue and sitespecific recombination in transgenic mice. This reaction is aided with the endonuclease activity of an enzyme. recombination is reversible, strategies for irreversible integration rely on mutated . * One unit is defined as the amount of enzyme necessary to produce maximal site-specific recombination of 0.25 g pLox2+ control DNA in 30 minutes at 37C in a total reaction volume of 50 l. Accessibility The schematic below shows the three types of rearrangements: inversion, deletion and translocation. These plasmids can be useful for lineage tracing, evaluating infection/transfection efficiency, selecting desired clones, and making transgenic mice. Wang DX, Qing SL, Miao ZW, Luo HY, Tian JS, Zhang XP, Wang SN, Zhang TG, Miao CY. Use of the system has greatly expanded . Migration of the branch point to the second cleavage site would then somehow trigger the second half of the pathway. 2. Simply put, the Cre/loxsystem rocks. Marth and Rajewsky reported in 1994 that Cre-lox recombination could be used for conditional gene targeting. The P1 bacteriophage uses these components as part of its natural viral life-cycle, and researchers have adapted the components for use in genome manipulation. These strains are designed to express a visible marker, such as green fluorescent protein (GFP) or LacZ, only after Cre recombinase excises a loxP-flanked stop sequence (Figure 7 and 8.). To create a conditional knock-out mouse, one needs the following systems: the Cre-loxP, and Applied Biological Materials - abm 40.7K subscribers Subscribe 1.4K 102K views 3 years ago The FLEx vector or "flip. When researchers started creating Cre recombinase mouse strains to generate tissue specific knockout mice, they needed an easy way to confirm that Cre recombinase was active only in certain tissues. place. It addresses the limitations of cre-loxP technology and related considerations for experimental design, and it discusses alternative strategies for site-specific genetic recombination and integration. Each site is 13 bp long, and an 8 bp . ADVERTISEMENTS: HHS Vulnerability Disclosure, Help Cre/loxP Recombination System: Applications, Best Practices - Taconic Methods for producing a maize plant by crossing hybrid maize variety X03R618CYFR with another maize plant are disclosed. instance, if two loxP sites are placed on different chromosomes, a translocation can take Here is the most efficient breeding scheme for generating Cre/loxtissue-specific or inducible knockouts. All cell types tested in mice have been shown to undergo transgenic Cre recombination. These sites, known as loxP sequences, contain specific binding sites for Cre that surround a directional core sequence where recombination can occur. Answer: a. As you can imagine, breeding such mice can get a wee bit complicated. Systems, Research 2001 Sep 21;505(3):467-73. doi: 10.1016/s0014-5793(01)02806-x. Cre activates gene of interest, using 4 mutated lox sites, Cre-loxP recombination is used to create a stochastic choice of expression among fluorescent proteins, resulting in the indelible marking of mouse neurons with multiple distinct colors, Multibow uses a binary code in which reporters are initially OFF and then probabilistically ON or OFF following Cre recombination to maximize label diversity, Rosa26 targeting vector, Cre dependent tdtomato expression. The Cre-Lox recombination system is derived from P1 Bacteriophages and uses a specific recombinase protein (Cre) that targets a unique DNA sequence (loxP) for splicing. conditional genetics; cre; cre-loxP; gene editing; loxP; recombination; site directed mutagenesis. 11. This patent application was filed with the USPTO on Wednesday, February 15, 2012 For We've encountered a problem, please try again. This system can be implemented without inserting any extra supporting proteins or sequences. You can read the details below. Cre-loxP-Mediated Recombination: General Principles and - PubMed This conserves the energy of the phosphodiester bond, allowing the reaction to be reversed without the involvement of a high-energy cofactor. We've updated our privacy policy. Initiation of site-specific recombination begins with the binding of recombination proteins to their respective DNA targets. 3. These developments have led to a widespread use of conditional mutagenesis in biomedical research, spanning many disciplines in which it becomes a powerful platform for determining gene function in specific cell types and at specific developmental times. The .gov means its official. mouse), and another with the loxP sites (the floxed mouse) are created, and crossed to. Studies have found that Cre recombinase is the best The temperature is 37 oC and Flp recombinase is 30. How do I prepare and deposit my plasmids? Cre-Lox recombination is commonly used to circumvent embryonic lethality caused by systemic inactivation of many genes. Conditional gene inactivation can be achieved with the Cre/ lox system in which the bacteriophage Cre recombinase promotes site-specific recombination at compatible loxP sites engineered in a gene of interest ( Sauer and Henderson, 1988 ). The Cre-lox system serves several functions in the phage: it circularizes the phage DNA into a plasmid, separates interlinked plasmid rings so they are passed to both daughter bacteria equally and may help maintain copy numbers through an alternative means of replication. Addgene: Cre-lox system Methods for producing a maize plant by crossing hybrid maize variety X00R808 with another maize plant are disclosed. If you run into any problems registering, depositing, or ordering please contact us at [emailprotected] It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. Cre-Lox recombination - Columbia University Postdoc Society Blog By expressing Cre at specific times or locations, you can precisely control expression of your gene of interest. US Patent for Maize hybrid X03R618CYFR Patent (Patent # 11,589,540 Mechanism of Cre-lox and FLP-FRT recombination Binding Synapsis , cleavage and strand exchange Three type of arrangement Inversion Translocation/ Insersion Deletion Application of Cre-lox and FLP-FRT recombination Disadvantage of FLP-FRT Advantage and disadvantage of Cre-lox Conclusion References KAUSHAL SAHU Follow Advertisement Advertisement Enhanced fungal resistance in transgenic cotton expressing an endochitinase g University Research Meeting Poster Apr 2014. Another report using two lox variants pair but through regulating the length of DNA in one pair results in stochastic gene activation with regulated level of sparseness. Using different lox site variants allows Cre to catalyze more than one specific recombination event in a single system. US Patent for Maize hybrid X00R808 Patent (Patent # 11,589,542 issued More novel and sophisticated uses for the Cre/loxsystem are being developed all the time. The Cre-lox system is used to introduce deletions, insertions, translocations, and inversions at a specific genomic locus. The system consists of two components derived from the P1 bacteriophage: the Cre recombinase and a loxP recognition site. Disclaimer. [28] Failure to activate both recombination events simultaneously confounds the interpretation of cell fate mapping results. You may not be able to create an account or request plasmids through this website until you upgrade your browser. RuvC is just one of these Resolvases that have been isolated in bacteria and yeast. the Flp-FRT system. b) The second step is supply of Cre recombinase. Cre-ERT2 - Tamoxifen inducible; Gateway entry vector, Cre expressed at low levels to reduce toxicity; See PI page for other recombinases that are less toxic in Drosophila, Cre-EGFP fusion; Tet inducible - rrTA expression driven by mouse Nkx cardiac enhancer and promoter fragment, N-terminal component of the Co-InCre system, C-terminal component of the Co-InCre system, Cre-ERT2; Targeting vector for Nanog locus, Cre, KASH-tagged EGFP, and sgRNA expression, DHFR-destabilized Cre; targeting vector for Rasgrf2, Cre-ERT2 - Tamoxifen inducible; Targeting vector, Cre fused to supernegatively charged GFP variant, Cre fused to the human Estrogen Binding Domain (EBD), Cre with a 25 nucleotide extracellular vesicle targeting sequence and CFP, mCherry and Cre expression in newborn neurons, GFP and Cre expression in newborn neurons, Cre recombinase dependent on GFP (CRE-DOG), iCre with MCS for inserting promoter, WPRE, CreER expression and tetracyclin-dependent transgene/shRNA expression, mCherry and Cre; expressed in excitatory neurons, For in vitro transcription of Cre or to recombine into BAC, TFP and Cre-ERT2 - Tamoxifen inducible. 10. Principle of Cre-DIO system - GeneMedi Recombination: Definition, Mechanism and Types | Microbiology Furthermore, recombination systems such as Cre-lox are widely used, mostly in eukaryotes. For a more thorough introduction, check out Addgenes Cre-lox blog post. Does Addgene accept orders by fax, phone or email? When cells that have loxP sites in their genome express Cre, a recombination event can occur between the loxP sites. Cre-lox System | Cyagen Korea A number of conserved site-specific recombination systems have been described in both prokaryotic and eukaryotic organisms. The Saccharomyces cerevisiae FLP-FRT recombination system is another site-directed recombination technology very conceptually similar to Cre-lox, with flippase (Flp) and the short flippase recognition target (FRT) site being analogous to Cre and loxP, respectively. Now customize the name of a clipboard to store your clips. 14.1k views Gene knockout samuel kwatia 52.1k views Knock out mice Priya Nanda Further cleavage of Holliday Intermediates results in formation of Hybrid DNA. Bookshelf A separate recombinase recognizes and binds to each of two recombination sites on two different DNA molecules or within the same DNA strand. The protein Cre recombinase recognizes 34 bp loxP sites, and the orientation and location of the loxP sites determines how the genetic material will be rearranged. We applied the Cre/lox recombinase-mediated cassette exchange (RMCE) system to Aedes aegypti, the vector of dengue, chikungunya, and Zika viruses. Maize hybrid X13C733 is an invention by Mario Rosario Carlone, Jr., Princeton IL UNITED STATES. If the Cre-lox system is incompletely knocking out a particular gene, remember that some genes are more easily recombined than others, possibly because the chromatin state is preventing Cre from accessing the loxP sites. Cre recombinase removes the stop sequence, so the transgene is expressed only in the cells where Cre is active (see Figure 4 below). Cre-Lox Recombination System & Conditional Mouse Models | Cyagen Linker, Adaptor, Homopolymeric Tailing & Terminal Transferase, Transcriptional and post transcriptional regulation of gene expression, Molecular biology of the gene ch 13 rna splicing part1, Alternative splicing : mechanism and regulation, POST TRANSCRIPTIONAL MODIFICATIONS IN EUKARYOTES, Nepalgunj Medical College and Teaching Hospital, govt .science college ,tumkur,( govt.estab), artificial or synthetic transcription factor for regulation of gene expression, Enzyme Kinetics and thermodynamic analysis, Chromatin, Organization macromolecule complex, eukaryotic translation machinery by kk sahu, Easy-handling carbon nanotubes decorated poly(arylene ether nitrile).pdf. Expressed in chondrocytes, mCherry and Cre-ERT2 - Tamoxifen inducible; Targeting vector for Pax7, Venus and Cre-ERT2 with MCS for inserting promoter, Emerald and Cre-ERT2 with MCS for inserting promoter, TFP and Cre-ERT2 with MCS for inserting promoter, tdTomato and Cre-ERT2 with MCS for inserting promoter, Venus, Cre-ERT2, targeting vector with MCS for homology arms, Emerald, Cre-ERT2, targeting vector with MCS for homology arms, TFP, Cre-ERT2, targeting vector with MCS for homology arms, tdTomato, Cre-ERT2, targeting vector with MCS for homology arms, GAG-Cre fusion; for the production of VLPs loaded with CRE protein, sfGFP-iCre-ERT2 (PAPGSTM N-terminus, unskippable linker) - Tamoxifen inducible, sfGFP-iCre-ERT2 (PAPGSTM N-terminus, GSAx9 linker) - Tamoxifen inducible, iCre-ERT2 (PAPGSTMA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PV N-terminus) - Tamoxifen inducible, iCre-ERT2 (PVV N-terminus) - Tamoxifen inducible, iCre-ERT2 (PVGSA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PA N-terminus) - Tamoxifen inducible, CreLite - Red light-inducible Cre; N terminus Cre fused to PIF6, CreLite - Red light-inducible Cre; C terminus Cre fused to PhyBdelta, CreLite system components, PhyBdeltaCreC and PIF6CreN, in middle entry vector (Tol2 kit), CreLite; Tol2 destination vector with mTagBFP2, CreLite system components, PhyBdeltaCreC and PIF6CreN, in AAV donor/transfer vector, CreLite system components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector, Cre recombinase split with Vivid photodimers, Cre-ERT2 - Tamoxifen inducible; AAV donor vector, iCre-ERT2 (PAA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAAA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAAAA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAGSA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAAGSA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAGSAS N-terminus) - Tamoxifen inducible, N-terminal Cre component split with Vivid photodimers, C-terminal Cre component split with Vivid photodimers, Split Cre fused to nuclear localized wild-type VVD, Retinal ganglion cell-specific expression of Cre, Cre activates shRNA expression, removal of EGFP; See also similar plasmids, Cre turns off shRNA expression, removal of EGFP; See also similar plasmids, Cre activates gene of interest by removing Stop sequences; Puro selection. You will have to adapt it to the genotypes you want to produce and the genetic backgrounds and characteristics of theloxP-flanked andcrestrains you use. [8] They observed 50% of the DNA polymerase beta gene was deleted in T cells based on DNA blotting. Cre-Lox recombination is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. In the absence of tamoxifen, CreER will result in the shuttling of the mutated recombinase into the cytoplasm. Therefore, inserting loxP sites at deliberate locations in a DNA sequence allows for very specific manipulations as discussed below. This has been largely due to the simplicity of its use and its adaptability to address diverse biological questions. a CAG promoter) and a loxP flanked stop cassette, ensuring the expression of the transgene is Cre-recombinase dependent and the reporter sequence. US Patent for Maize inbred 2ZZSB1121 Patent (Patent # 11,589,535 issued The 13 bp sequences are palindromic but the 8 bp spacer is not, thus giving the loxP sequence a certain direction. PubMed CentralPMCID:PMC49604. MeSH Plasmid, Cre activates your gene fused to eGFP, removes dsRed. Use of the system has greatly expanded our ability to precisely interrogate gene function in the mouse, providing both spatial and temporal control of gene expression. Both 13bp repeat sequences on a single loxP site are recognized and bound by aCre protein, forming a dimer. PMC DNA Replication in eukaryotes and prokaryotes. called the FLP (pronounced 'flip') protein. [30] Importantly, sometimes fluorescent reporters can be activated in the absence of tamoxifen, due to leakage of a few Cre recombinase molecules into the nucleus which, in combination with very sensitive reporters, results in unintended cell labelling. Shortly thereafter, researchers in the laboratory of Prof. Klaus Rajewsky reported the production of pluripotent embryonic stem cells bearing a targeted loxP-flanked (floxed) DNA polymerase gene. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR, AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR, AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR, AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA, pGEMT-Pax7bait-P2a-GAP43-Cherry-T2a-mERt-Cre-Ert, pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2, pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre, hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean, pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP, Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientists View, CREM and CMV-STOP-luc cassette, separated by insulator, Cre-EGFP fusion in a promoterless vector with several available restriction sites, tet inducible Cre-GFP fusion, metallothionein MT-I region including the polyadenylation site and several introns, Cre-ERT2 with loxp cassette; Targeting vector for Rosa26 locus, CREM fused to mutant AR ligand binding domain, Cre fused to HIV-TAT - promotes cellular uptake of recombinant Cre, Cre-Myc, Expressed in rod photorecetor cells in the retina. Cre-lox Systems Cre-lox (floxed) systems for gene deletion ( click here for non-floxed and earlier floxed tagging vectors) General Comments The baseline reference for this system is CRE recombinase-based positive-negative selection systems for genetic manipulation in Trypanosoma brucei (2008) Scahill MD, Pastar I & Cross GAM. Is LysM-Cre a good candidate Cre for knocking out Atg5 gene in mice. FLP-FRT & Cre-lox Recombination Homologous and Site-Specific Recombination - [PPT Powerpoint]

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